The solid phase extraction column is a sample pretreatment device developed from a chromatography column for extraction, separation and concentration.
It is mainly used for sample pretreatment of target compounds in various food, agricultural and animal products, environmental samples and biological samples. Solid phase extraction technology is widely used in many national standards (IOS) and industry analysis standards.
The capacity of the solid phase extraction column refers to the adsorption capacity of the packing of the solid phase extraction column. For silica-based solid phase extraction columns, the capacity is generally 1 to 5 mg/100 mg, ie. H. the column capacity is 1 to 5% of the packing mass.
The capacity of the bound silica gel ion exchange adsorbent filler is expressed in meq/g, i.e. H. the capacity per gram of filler is X milligram equivalentsn. The capacity of this type of filler is normally 0.5 to 1.5 meq/g.
SPE technology is based on the theory of liquid-solid phase chromatography and uses selective adsorption and selective elution to enrich the sample, Separation and purification is a physical extraction process that includes liquid phase and solid phase; it can also be viewed as a simple chromatographic process.
SPE is the separation principle of liquid chromatography using selective adsorption and selective elution.
The most commonly used method is to pass the liquid sample solution through the adsorbent, retain the substance to be tested, then use a suitable strength solvent to wash away the impurities, and then remove them to use a small amount of solvent to quickly elute the substance to be tested. To the purpose of a beautifulto achieve all separation, purification and concentration.
It can also selectively adsorb interfering impurities and emit the measured substance; or simultaneously adsorb impurities and the measured substance, and then use an appropriate solvent to selectively elute the measured substance.